Functionalization of cerium oxide nanoparticles by simple biomolecules

In this diploma thesis we propose to study nanoparticles of cerium dioxide with a size of a few nanometers functionalized by simpler biomolecules, such as histidine or glycine, from solution. The nanoparticles will be characterized by microscopic methods (AFM, SEM, TEM), dynamic light scattering (DLS) and UV-vis spectrometer. The focus will be on the study of the nature of bonds formed between biomolecules and CeO₂ nanoparticles by photoelectron spectroscopy using synchrotron radiation (SRPES, RPES, NEXAFS) and XPS. The research will be carried out in collaboration with Dr. A. Shcherbakova and Dr. N. Zholobakova, Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine. The photoemission experiments will be carried out mainly at the Materials Science Beamline (MSB) at the Elettra synchrotron in Trieste. The aim of this work will be to study the way biomolecules interact with the surface of cerium dioxide nanoparticles.

Literature

1. Catalysis by Ceria and Related Materials, A. Trovarelli, Imperial College Press, ISBN: 1-86094-299-7.
2. D. Briggs, M.P. Seah: Practical Surface Analysis, vol. 2 - Auger and X-ray Photoelectron spectroscopy, Wiley, 1990, ISBN 0-471-92081-9
3. N. Hashemi Goradel, F. Ghiyami-Hour, S. Jahangiri, B. Negahdari, A. Sahebkar, A. Masoudifar, H. Mirzaei, Nanoparticles as new tools for inhibition of cancer angiogenesis, J. Cell. Physiol. 233 (2018) 2902–2910
4. C.D. Spicer, E.T. Pashuck, M.M. Stevens, Achieving Controlled Biomolecule–Biomaterial Conjugation, Chem. Rev. 118 (2018) 7702–7743
5. A. Dadwal, A. Baldi, R. Kumar Narang, Nanoparticles as carriers for drug delivery in cancer, Artif. Cells, Nanomedicine, Biotechnol. 46 (2018) 295–305
6. Y. Lin, J. Ren, X. Qu, Catalytically active nanomaterials: a promising candidate for artificial enzymes, Acc. Chem. Res. 47 (2014) 1097–1105
7. X. Wang, Y. Hu, H. Wei, Nanozymes in bionanotechnology: from sensing to therapeutics and beyond, Inorg. Chem. Front. 3 (2016) 41–60
8. S.P. Pujari, L. Scheres, A.T.M. Marcelis, H. Zuilhof, Covalent Surface Modification of Oxide Surfaces, Angew. Chemie Int. Ed. 53 (2014) 6322–6356
9. P.R. Solanki, A. Kaushik, V. V Agrawal, B.D. Malhotra, Nanostructured metal oxide-based biosensors, NPG ASIA Mater. 3 (2011) 17–24
10. R.W. Tarnuzzer, J. Colon, S. Patil, S. Seal, Vacancy engineered ceria nanostructures for protection from radiation-induced cellular damage., Nano Lett. 5 (2005) 2573–7.
11. I. Celardo, J.Z. Pedersen, E. Traversa, L. Ghibelli, Pharmacological potential of cerium oxide nanoparticles, Nanoscale. 3 (2011) 1411–1420
12. R. G. Acres, X. Cheng, K. Beranová, S. Bercha, T. Skála, V. Matolín, Y. Xu, K. C. Prince, N. Tsud: An experimental and theoretical study of adenine adsorption on Au(111), Phys. Chem. Chem. Phys. 20 (2018) 4688 – 4698
13. S. Bercha, K. Beranová, R. G. Acres, M. Vorokhta, M. Dubau, I. Matolínová, T. Skála, K. C. Prince, V. Matolín, N. Tsud: Thermally Controlled Bonding of Adenine to Cerium Oxide: Effect of Substrate Stoichiometry, Morphology, Composition, and Molecular Deposition Technique, J. Phys. Chem. C, 121 (2017) 25118-25131
14. N. Tsud, R.G. Acres, M. Iakhnenko, D. Mazur, K.C. Prince, V. Matolín, Bonding of Histidine to Cerium Oxide, J. Phys. Chem. B 117 (2013) 9182-9193
15. N. Tsud, S. Bercha, R. G. Acres, M. Vorokhta, I. Khalakhan, K. C. Prince, V. Matolín: Functionalization of nanostructured cerium oxide films with histidine, Phys. Chem. Chem. Phys. 17 (2015) 2770 – 2777
16. R. G. Acres, V. Feyer, N. Tsud, E. Carlino, K. C. Prince: Mechanisms of Aggregation of Cysteine Functionalized Gold Nanoparticles, J. Phys. Chem. C. 118 (2014) 10481-10487